Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Matt,

for which type of processing do you need the raw overlay? Overlays are 
usually done by the software and not saved (at least not in raw format).

If I remember correctly, you can export the overlay from the experiments 
list as ordinary tif or jpg. I am not sure about LCS, but it works in 
LAS AF.

Michael


Matthew Pearson wrote:
> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi Everyone,
> 
> Does anyone know why in the Leica Confocal Software when you scan 
> multiple fluorophores you select the 'Ovl' button to get an overlay 
> image and then you would press the Single scan button to take an image.  
> Yet in the Experiment window where the files are saved the overlay image 
> is not saved only the 3 images of each fluorophore (in a triple label).  
> I have to actually right click the overlay image  and then 'send to 
> experiment, selection raw' in order for it to be saved.  does anyone 
> know if there is a setting to allow the overlay image to be saved 
> without having to save each one manually?  It just seems like a slightly 
> tedious process.
> 
> Many thanks,
> 
> Matt Pearson.
> 
> 
> -- 
> Imaging Technician
> Cell Biology Division
> Institute of Ophthalmology
> University College London
> EC1V 9EL
> 020 7608 6857
> [log in to unmask]