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Dear Vaishali,
I'd suggest to check whether your slides are hydrophobic or
hydrophylic. Cells usually don't like hydrophobic ("dirty") glass
slides. A short (oxygen) plasma treatment could help. Of course there
are also many solvent based cleaning protocols around.
Cheers Gabor
--
Gabor Csucs
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16
CH-8093, Zurich, Switzerland
Web: www.lmc.ethz.ch
Phone: +41 44 633 6221
Fax: +41 44 632 1298
e-mail: [log in to unmask]