Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

BTW, if you are using an upright microscope, you can grow the cells 
on LabTek culture-well slides.  Get the RS, which has a coating that 
resembles tissue culture plastic.
Carol


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear All
>We are working with B16F10 melanoma cell line  which
>is a highly adhesive line , recently we are facing the
>problem of growing them . When we grow them in petri
>plates it spreads very well but when seeded on
>coverslips it remains rounded. What could be the
>possible reason.
>Regards
>Vaishali
>
>
> 
>____________________________________________________________________________________
>You rock. That's why Blockbuster's offering you one month of 
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-- 
Carol A. Heckman, Ph.D.
Director, Center for Microscopy & Microanalysis
and Professor of Biological Sciences
Bowling Green State University
Bowling Green, OH 43403
USA
website: http://www.bgsu.edu/departments/biology/facilities/MnM