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They probably don't like to grow on glass and prefer plastic. You could try different adherence coatings to be able to grow them on glass.
Monique Vasseur
Microscopie et imagerie
Département de biochimie
Université de Montréal
C.P. 6128, succursale Centre-ville
Montréal QC H3C 3J7 Canada
tél. (514) 343-6111 poste 5148
-----Message d'origine-----
De : Confocal Microscopy List [mailto:[log in to unmask]] De la part de vaishali kailaje
Envoyé : 3 avril 2008 06:19
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Objet : Not a confocal question
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Dear All
We are working with B16F10 melanoma cell line which
is a highly adhesive line , recently we are facing the
problem of growing them . When we grow them in petri
plates it spreads very well but when seeded on
coverslips it remains rounded. What could be the
possible reason.
Regards
Vaishali
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