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Hi 

A problem with FDA it can also diffuse out of the cell after cleavage
causing the surrounding media to fluorese, fungal material diffuses in
cytosol, and leaves the nulear material less bright.

Russ

Russell N. Spear
Sr. Research Specialist
Dept. of Plant Pathology
Univ. of Wisconsin
1630 Linden Dr.
Madison WI 53706

voice 608.263.2093
fax     608.263.2626

>>> F Javier Diez Guerra <[log in to unmask]> 04/08/08 9:44 AM >>>
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Hi,

anybody knows if FDA (fluorescein diacetate) 
distributes evenly within cells once incorporated 
and cleaved by cellular esterases?

I assume that once cleaved, FDA can no longer 
cross membranes and therefore remains trapped in soluble compartments.

In other words, can FDA be used as a marker of 
internal cell volume due to its free diffusion 
among continuous cellular compartments (Vg. cytosol + nuclear matrix)?

Thanks


F Javier Diez-Guerra, PhD
Profesor Titular
Centro de Biologia Molecular Severo Ochoa
C/ Nicolás Cabrera, 1
Universidad Autónoma
Ctra Colmenar Viejo Km 15
Cantoblanco, 28049 Madrid
SPAIN

phone:  +34 91 196 4612
e-mail: [log in to unmask]