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Yes, the cleaved product is moderately membrane-permeable over time,
especially if the cells are strongly loaded with the dye, but you can use
one of the larger, and less membrane-permeable relatives, such as CFDA -
there are others as well.

cheers,
Rosemary

Rosemary White                    [log in to unmask]
CSIRO Plant Industry            ph.     61 (0)2-6246 5475
GPO Box 1600                       fax.     61 (0)2-6246 5334
Canberra, ACT 2601 
Australia



On 9/4/08 1:51 AM, "Russell Spear" <[log in to unmask]> wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hi 
> 
> A problem with FDA it can also diffuse out of the cell after cleavage
> causing the surrounding media to fluorese, fungal material diffuses in
> cytosol, and leaves the nulear material less bright.
> 
> Russ
> 
> Russell N. Spear
> Sr. Research Specialist
> Dept. of Plant Pathology
> Univ. of Wisconsin
> 1630 Linden Dr.
> Madison WI 53706
> 
> voice 608.263.2093
> fax     608.263.2626
> 
>>>> F Javier Diez Guerra <[log in to unmask]> 04/08/08 9:44 AM >>>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Hi,
> 
> anybody knows if FDA (fluorescein diacetate)
> distributes evenly within cells once incorporated
> and cleaved by cellular esterases?
> 
> I assume that once cleaved, FDA can no longer
> cross membranes and therefore remains trapped in soluble compartments.
> 
> In other words, can FDA be used as a marker of
> internal cell volume due to its free diffusion
> among continuous cellular compartments (Vg. cytosol + nuclear matrix)?
> 
> Thanks
> 
> 
> F Javier Diez-Guerra, PhD
> Profesor Titular
> Centro de Biologia Molecular Severo Ochoa
> C/ Nicolás Cabrera, 1
> Universidad Autónoma
> Ctra Colmenar Viejo Km 15
> Cantoblanco, 28049 Madrid
> SPAIN
> 
> phone:  +34 91 196 4612
> e-mail: [log in to unmask]