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>


Do they offer the option of violet (or other) lasers in addition to  
the supercontinum laser? That would extend it beyond the 470-670 limits?

The brochure also mentions cooled PMTs - I wonder what real-world  
impact that will have?

Regards,

Adrian Smith
Centenary Institute, Sydney, Australia



>
> Just a correction .........
> Leica SP5X tunable white light laser system covers 470-670 nm only,  
> not the entire visible spectrum. Very stable, provide 1.5 mW per nm,  
> need more power... increase the bandwidth.
> ammasi
>
>
>
> On Mon, 31 Mar 2008 21:55:26 -0500
> Mayandi Sivaguru <[log in to unmask]> wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> Page, check out also the Leica spectral excitation and spectral  
>> emission confocal SP5 X. This has a single white laser with a  
>> single laser capable of tuning excitation wavelengths at the entire  
>> visible spectrum at 1nm resolution.
>> In addition, remember the SP5 comes with a resonant scanner too. I  
>> have personally tested a SP5 system with STED with the samples I  
>> have prepared and very pleased with the results. That means if you  
>> have a SP5 with resonant scanner you can latter upgrade to  
>> superresolution technique (STED) which can yield around 90 nm  
>> resolutions in xy. THe AOBS based system is great as you loose less  
>> photons.
>> But, the Zeiss 710 system's new grating design is worth to have a  
>> look. I personally feel that the Zeiss 710 is a system step cloaser  
>> to a spectral confocal such as an SP5. Zeiss has great flexibility  
>> in your laser selection and that will let you not burn your wallet  
>> upfront and you can add lasers latter as and when it is needed.
>> No commercial interest on either vendors. I feel both should be  
>> great systems and you should select depending on your necessity and  
>> your budget.
>> Shiv
>> At 02:40 PM 3/31/2008, you wrote:
>>> Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>> Hi Page,
>>>
>>> We had a new SP5 installed mid-August 2007 and we have been happy  
>>> with it. The system had the usual teething problems, but Leica   
>>> was rather efficient in fixing them.  The software was never  
>>> Leica's strong side, and the new LAS AF is no exception, but you  
>>> learn to live with it; the 1.8.2 version is definitely an  
>>> improvement.  The PMTs are great with a wonderful STN ratio, and  
>>> the objectives are very good as well (the system shows practically  
>>> no lateral or axial chromatic aberration, e.g.). I don't know the  
>>> 710.
>>> Hope this helps!
>>>
>>> Zoltan
>>>
>>> On Mon, Mar 31, 2008 at 7:28 PM, D. Page Baluch <<mailto:[log in to unmask] 
>>> >[log in to unmask]> wrote:
>>> Search the CONFOCAL archive at <http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal 
>>> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal We are  
>>> looking to upgrade our facility with a new confocal system. For  
>>> the past 10 years we have used both the Leica TCSNT and SP2  
>>> systems and have been quite satisfied with their performance. We  
>>> are now leaning towards the Leica SP5 but we would like to hear  
>>> from others about their experiences with other confocal systems  
>>> and why they think one might be better than another. We recently  
>>> saw a demo of the Zeiss LSM 710 which appears to be a good system  
>>> and know of some researchers who have used the 510 and love it.  
>>> Most of our users have fixed samples and use an upright microscope.
>>> Thanks for your suggestions and advice,
>>> Page
>>>
>>> --
>>> Page Baluch, Ph.D.
>>> W.M. Keck Bioimaging Lab Manager
>>> Arizona State University/SoLS
>>> PO Box 874501
>>> Tempe, AZ 85287-4501
>>>
>>>
>>>
>>>
>>> --
>>> --
>>> Zoltan Cseresnyes
>>> Facility manager, Imaging Suite
>>> Dept. of Zoology University of Cambridge
>>> Downing Street, Cambridge
>>> CB2 3EJ UK
>>>
>>> Tel.: (++44) (0)1223 769282
>>> Fax.: (++44) (0)1223 336676
>> Mayandi Sivaguru, PhD, PhD
>> Microscopy Facility Manager
>> 8, Institute for Genomic Biology
>> University of Illinois at Urbana-Champaign
>> 1206 West Gregory Dr.
>> Urbana, IL 61801 USA
>> Office: 217.333.1214
>> Fax: 217.244.2496
>> [log in to unmask]
>> http://core.igb.uiuc.edu
>
> Ammasi Periasamy, Ph.D.
> Director, Keck Center for Cellular Imaging (KCCI)
> Professor of Biology and Biomedical Engineering
> Biology, Gilmer Hall (064), McCormick Rd
> University of Virginia
> Charlottesville, VA 22904
> Voice: 434-243-7602 (Office); 982-4869 (lab)
> Fax:434-982-5210; Email:[log in to unmask]
> http//:www.kcci.virginia.edu
> ************************
> Workshop on FRET Microscopy, March 3-7, 2009
> http://www.kcci.virginia.edu/workshop/workshop2009/index.php
> *************************