Subject: | |
From: | |
Reply To: | |
Date: | Wed, 16 Apr 2008 08:56:41 +0200 |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hello Cam,
yes, Imaris is able to split tracks and follow daughter cells - also vice
versa. How well this works depends mainly on your dataset. With a rather
high temporal resolution (cells move less than their diameter between two
frames) and well defined cells (spatial resolution, signal-to-background)
it shouldn't be a problem. If you would like to go this way, I recommend
to directly ask your local Bitplane representative to give you an
introduction.
Michael
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi Everyone,
>
> As is always the way with these things, now
> i have had a bit more of a think about it i have some more information
> to add to my request. With the tracking we want to be able to track a
> field of cells. If one of the cells divide we want to be able to track
> the resulting daughter cells. I have used imaris in the past but i don't
> remember it having the capacity to recognise a cell has divided and set
> up individual traces for the resulting daughter cells. Can imaris (or
> any of the other software products mentioned by others) do this specific
> function? And if so how reliable are they at doing it.
>
>
>
> Thanks
>
>
>
>
>
> Cam
>
>
>
>
>
>
>
>
>
>
>
> Cameron Nowell
> Microscopy Research and Imaging Facility
> Cell Cycle and Development
> Peter MacCallum Cancer Centre
> 7 St Andrews Place
> East Melbourne, 3002
> Victoria AUSTRALIA
>
> Phone: +61396563759
> Fax: +61396561411
> Mobile: +61422882700
>
>
>
>
>
> This email (including any attachments) may contain
> confidential and/or legally privileged information and is
> intended only to be read or used by the addressee. If you
> are not the intended addressee, any use, distribution,
> disclosure or copying of this email is strictly
> prohibited.
> Confidentiality and legal privilege attached to this email
> (including any attachments) are not waived or lost by
> reason of its mistaken delivery to you.
> If you have received this email in error, please delete it
> and notify us immediately by telephone or email. Peter
> MacCallum Cancer Centre provides no guarantee that this
> transmission is free of virus or that it has not been
> intercepted or altered and will not be liable for any delay
> in its receipt.
>
>
>
>
> --
> Chris Tully
> Microscopy and Image Analysis Expert
> [log in to unmask]
> 240-888-1021
> http://www.linkedin.com/in/christully
>
>
>
> This email (including any attachments) may contain
> confidential and/or legally privileged information and is
> intended only to be read or used by the addressee. If you
> are not the intended addressee, any use, distribution,
> disclosure or copying of this email is strictly
> prohibited.
> Confidentiality and legal privilege attached to this email
> (including any attachments) are not waived or lost by
> reason of its mistaken delivery to you.
> If you have received this email in error, please delete it
> and notify us immediately by telephone or email. Peter
> MacCallum Cancer Centre provides no guarantee that this
> transmission is free of virus or that it has not been
> intercepted or altered and will not be liable for any delay
> in its receipt.
|
|
|