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Dear Tina,
Do you think that your guidelines are sufficient? If someone is allowed
to change the brightness/contrast and gamma settings for the different
fluorescent channels separately and save those "new", edited images
(especially if I do repeated cycles of this procedure) - it is pretty
easy to create completely new images - showing e.g. more or less
co-localization. The problem is that also the above mentioned procedures
are able to manipulate the information content of an image... (OK they
are always just reducing it - but still, one could use them to "cut out"
the unwanted stuff). Don't you think so?
Greetings Gabor
--
Gabor Csucs
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16
CH-8093, Zurich, Switzerland
Web: www.lmc.ethz.ch
Phone: +41 44 633 6221
Fax: +41 44 632 1298
e-mail: [log in to unmask]