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June 2008

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From:
Csucs Gabor <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 23 Jun 2008 23:17:47 +0200
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Dear Tina,

Do you think that your guidelines are sufficient? If someone is allowed 
to change the brightness/contrast and gamma settings for the different 
fluorescent channels separately and save those "new", edited images 
(especially if I do repeated cycles of this procedure) - it is pretty 
easy to create completely new images - showing e.g. more or less 
co-localization. The problem is that also the above mentioned procedures 
are able to manipulate the information content of an image... (OK they 
are always just reducing it - but still, one could use them to "cut out" 
the unwanted stuff). Don't you think so?

Greetings   Gabor

-- 
Gabor Csucs 
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16 
CH-8093, Zurich, Switzerland

Web: www.lmc.ethz.ch
Phone: +41 44 633 6221
Fax: +41 44 632 1298
e-mail: [log in to unmask]

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