Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

The light path on the SP5 goes to the side port via the scan mirrors 
but not the spectral separation path. Going via the scan mirrors allows 
you to place the beam at a series of locations when collecting a set of 
FCS data (though you could do this by moving the stage with a simply 
parked beam I suppose). The set-up we have uses a dichroic and barrier 
filters to separate emission bands to two APDs. It works well, but, of 
course, you have to change the filter / mirror combinations if you 
change your cross-correlation fluorophores, but that is pretty easy too.

IAN


On Thursday, June 19, 2008, at 02:45  AM, Jean-Pierre CLAMME wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hi,
>
> First thank you for the infos.
>
> Second do you need to go back through the scan heads ? Is it possible 
> to use another side port to extract the light for example to do cross 
> correlation ? I worked on two home made systems (FCS + imaging and 
> single molecule) were we would collect the light directly  after the 
> objective and redirect the signal to multiple APDs. On the SP5 we have 
> here we have external detectors. Could that kind of approach  be used > ?
>
> Thanks
>
> JP
>
> Michael Weber wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Jean-Pierre,
>>
>> on both systems one needs to install the external port modification 
>> (X1 / port 4 option), in order to bring the light out of the 
>> scan-head. Beside that, it's just another discussion about which 
>> system offers better transmission - dichromatic mirrors or AOBS.
>>
>> Gabor, can you control the APDs for imaging via the Leica software, 
>> or do you have to use an external solution?
>>
>> Michael
>>
>>
>> Jean-Pierre CLAMME wrote:
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>>
>>> Hi,
>>>
>>> I'm wondering if onyone has experience with modifying a SP5 or a 
>>> Fluoview
>>> 1000 for FCS mesures ? I work with Olympus stands before and they 
>>> generally
>>> offer good access to external ports. But what a bout the SP5 ?
>>> Thanks
>>>
>>> JP
>>
>>
>
> -- 
> Dr. Jean-Pierre CLAMME
> Supervisor 2-Photon Imaging Facility Dept. of Immunology, IMM-1, R310
> The Scripps Research Institute
> 10550 North Torrey Pines Rd.
> La Jolla, California 92037
>
> Lab number: (858)784-8184
> Fax number:(858)784-9272
>
>

* * * * * * * * * * *
Prof Ian Gibbins
Anatomy & Histology
Flinders University
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