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We've measured that and it's negligible under our usual conditions, 
which is a relief...

IAN


On Thursday, June 19, 2008, at 08:54  AM, Jean-Pierre CLAMME wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> This is what we were doing on our system. We would choose  a point and 
> then move the table to bring that point under the parked beam.
> I'm just wondering, is the fact  that scanning moves the beam  
> changing the focal volume from one point to the other ? Or is it 
> negligible ?
>
> Thanks
>
> JP
>> The light path on the SP5 goes to the side port via the scan mirrors 
>> but not the spectral separation path. Going via the scan mirrors 
>> allows you to place the beam at a series of locations when collecting 
>> a set of FCS data (though you could do this by moving the stage with 
>> a simply parked beam I suppose).
> This is what we were doing on our system. We would choose  a point and 
> then move the table to bring that point under the parked beam.
> I'm just wondering Is the fact that when you use the scanner, you  but 
> the fact that when you use the scanner,
>
>
>>
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>>
>> http://som.flinders.edu.au/FUSA/Anatomy/
>> http://www.flinders.edu.au/neuroscience
>>
>
>

* * * * * * * * * * *
Prof Ian Gibbins
Anatomy & Histology
Flinders University
GPO Box 2100
Adelaide SA 5001
AUSTRALIA

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