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I am a PhD student at Auburn University just getting started into my thesis work. I am
looking for a reliable method for quantifying fluorescence.
My project deals with marine dinoflagellates (zooxanthellae) that reside intracellularly in
hosts (usually cnidarians). I am working with cultures or isolates of only the dinoflagellates
for my confocal work. I have an antibody that was created against the surface secretions
of mucilage (secreted as part of a daily cycle by the alga) from one strain of zooxanthellae.
I am attempting to use this antibody to label various strains to identify differences in
mucilage between them. Thus far, I have seen that the strain the antibody was created
against labels around the cell fairly brightly. Most samples either show this or a complete
lack of labeling. However, a few samples show a faint fluorescence lifted off the cell
surface. I would like to be able to quantify this fluorescence in comparison to either the
control strain that the antibody was created against or against a known fluorescence.
Any help on ideas for this, or places to look for ideas would be greatly appreciated.
Thanks!
Maria Mazzillo