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Dear all,

I am practice FRAP on Zeiss LSM 510-META.

Right now I do know how to induce the bleach and record the recovery curve 
of ROI(by using click "MeanROI" in time series control ). But everytime at the 
end of recording, only the curves are saved. So my question is can I save the 
series of image data as well as the  curve?

Another question is after induce bleach I need to restart the scanning and 
draw the ROI to measure the mean intensity of them. I always worry about 
that I might miss the ROI where bleaching happens. Does anyone here know 
how to do it in a proper way? 

I really appreciate any help!

Thanks,


Yi