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July 2008

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Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 24 Jul 2008 09:48:28 -0500
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Vitaly Boyko <[log in to unmask]>
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NCI-Frederick, HIV-DRP
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Vitaly Boyko <[log in to unmask]>
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Dear All,

Yesterday I have tested our new 585 nm laser in conjuction with 514 nm argon 
and 437 nm diode under TIR conditions.

Even though I have not noticed the need for the critical angle re-adjustment 
between 437 nm and 514 nm lines (77 nm difference), however, when the 
critical angle was set for the yellow laser (585 nm), switching from 585 nm 
to 514 nm line  (71 nm difference) required the (manual) resetting of the 
critical angle.
I used the 89006 triple beamsplitter (A/R coated), which seemed to reflect 
the 514 argon line (up to 70%).

I am looking for help with the device that would allow quick, low msec 
range, critical angle re-adjustment or any other suggestions will be 
appreciated.

Thanks a lot in advance,

Vitaly

Vitaly P. Boyko,
NCI-Frederick,
301-846-6575


 

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