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Dear Yi,

You cannot directly save the curve -except you want to work on a
screenshot- but you can select to show a table containing the plotted
values, these values can be exported to another software to regenerate
the graph.

There is a 'ROI' folder somewhere in the AIM tree containing the
position coordinates of the ROIs you generated. To read data from the
bleached region, you can recall the ROI from a list as described by
Leoncio.

Just as a FRAP aside: be careful with the bleaching power. You can
easily achieve powers sufficient to influence molecular interactions
with a standard 15mW laser...

Cheers, jens

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Vergara, Leoncio A.
Sent: Wednesday, July 23, 2008 9:35 PM
To: [log in to unmask]
Subject: Re: Question about LSM510 software

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I am not sure if there is a way to save both the curve and the images,
although it would be nice and useful to see the curve in real time, I
usually do the FRAP experiments in our LSM510 meta just saving the
images, I look at the curve off line, its very easy to reload the images
and use the MeanROI to get the curve from the saved images.

regarding savin g the ROI you used for bleaching, there is an option to
save the ROI from the ROI editing window. You can always retrieve it
later when using the Mean ROI function... just look at the ROI listing.

Leoncio



________________________________________
From: Confocal Microscopy List [[log in to unmask]] On Behalf
Of Yi Zheng [[log in to unmask]]
Sent: Wednesday, July 23, 2008 1:39 PM
To: [log in to unmask]
Subject: Question about LSM510 software

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Dear all,

I am practice FRAP on Zeiss LSM 510-META.

Right now I do know how to induce the bleach and record the recovery
curve
of ROI(by using click "MeanROI" in time series control ). But everytime
at the
end of recording, only the curves are saved. So my question is can I
save the
series of image data as well as the  curve?

Another question is after induce bleach I need to restart the scanning
and
draw the ROI to measure the mean intensity of them. I always worry about
that I might miss the ROI where bleaching happens. Does anyone here know
how to do it in a proper way?

I really appreciate any help!

Thanks,


Yi