Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
However, taking into account all these factors, an MP microscope
is 'perfectly' confocal, whereas no 'real' confocal microscope
can ever be. (Unless you have some way of making an infinitely
small pinhole.) See:
Guy Cox & Colin Sheppard, 2004. Practical limits of resolution in
confocal and non-linear microscopy. Microscopy Research & Technique,
63, 18-22
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of George McNamara
Sent: Monday, 28 July 2008 9:30 AM
To: [log in to unmask]
Subject: Re: Classification of confocal microscopes
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi Juan,
confocal refers to conjugate pinhole. So on a MP-only microscope with non-descanned detector(s): no.
However, most MP systems are mounted on a confocal microscope and the confocal detectors are often used instead of the NDD's. The confocal detector pinhole(s) are usually adjustable (typically to 1 Airy unit, based on the objective lens NA), but even a "wide open" pinhole may be much much smaller than if there was no pinhole aperture diaphragm in place (an NDD condition!).
So, most MP microscopes are multiphoton+confocal.
When writing the methods section, be sure to specify what light path is used and whether using NDD or confocal detector, and if the latter, the number of Airy units for the pinhole size (there are no laws forcing you to use 1 Airy unit, in fact 2 Airy units will typically double the amount of light collected).
George
p.s. on an MP system, the confocal detectors reject stray light, so if the room is not dark (an MP scope needs to have a black shroud!) the confocal detectors will usually have a lower background than the NDD's. A simple test is to shine a flashlight at the specimen while scanning with either.
At 04:27 AM 7/23/2008, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear all,
>It's just a theoretical question. Can
>multiphoton microscopes be classified as confocal microscopes?
>Is there any reference published about this type of microscope classification?
>
>Thank you very much in advance
>
>--
>Juan Luis Ribas
>Servicio de Microscopía
>Centro de Investigación, Tecnología e Innovación Universidad de Sevilla
>Av. Reina Mercedes 4b
>41012 Sevilla
>
>Tfno: 954559983
George McNamara, Ph.D.
University of Miami, Miller School of Medicine Image Core Miami, FL 33010 [log in to unmask] [log in to unmask]
305-243-8436 office
http://home.earthlink.net/~pubspectra/
http://home.earthlink.net/~geomcnamara/
http://www.sylvester.org/research/SR_lab_analytical.asp?ana=desc
(Analytical Imaging Core Facility)
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