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Hello there,

the DiI / DiO related membrane stain DiD from 
Molecular Probes comes in two flavors (as do its relatives):

'DiD' oil = DiIC18(5) oil (cat# D307) or as 'DiD' 
solid = DiIC18(5) solid (cat# D7757).

Can anyone comment on what the 
advantages/disadvantages of the two flavors are?

The idea for the experiment is to use DiD to 
stain the membranes of endothelial cells of blood 
vessels (from the interior of the vessel), to be 
able to distinguish the endothelial cells better 
from neighboring smooth muscle cells (SMCs) 
Povided that the endothelium is intact, the dye 
should not be able to reach the SMCs - or so ist 
the hope. DiD should be sufficiently in the red 
to allow the simultaneous usage of the green 
channel e.g. for Fluo-4 Ca measurements.

Regards

Steffen

-- 
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)

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