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November 2008

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Subject:
From:
Glen MacDonald <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 19 Nov 2008 12:13:59 -0800
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Many aquatic invertebrates have autofluorescence spanning the spectrum  
from UV to red. Also, a DAB immunolabeled tissue slide dipped briefly  
in glutaraldehyde and rinsed before coverslipping will display green- 
yellow fluorescence with dark DAB deposits.

Glen
On Nov 19, 2008, at 11:41 AM, Jonathan M Krupp wrote:

> On Tue, 18 Nov 2008 19:46:15 -0700
> John Chandler <[log in to unmask]> wrote:
>> So, Jon,
>> Which demo samples do you like?  Which ones have good effort to  
>> results ratio?
>> Inquiring minds want to know.
>
>
> I'll get a more comprehensive list together and send it to you, but  
> for now here's what I have tried.
>
> Simple H&E sections are amazingly useful. I didn't believe it when  
> someone suggested it, but they really work to demo fluorescence in a  
> simple way.
>
> I am going to try pollen and some freehand sections of some local  
> plants asap. Also looks like the prepared test slides from  
> Invitrogen might be a good bet if you can afford them.
>
> Jon

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