Hi,

I am currently using linear unmixing on the the Zeiss 510 Meta confocal 
microscope to unmix the background autofluorescence from specific 
fluorescence in parrafin sections.

I am using the 488nm laser, and the results are very varied depending on the 
tissue type I am imaging.

Has anybody tried linear unmixing using the 488nm laser and produced good 
images? Is there anything I can do to acquire better images?

Any help would be very much appreciated, thanks!