samrina aslam wrote:
> Hi,
>
> I am currently using linear unmixing on the the Zeiss 510 Meta confocal 
> microscope to unmix the background autofluorescence from specific 
> fluorescence in parrafin sections.
>
> I am using the 488nm laser, and the results are very varied depending on the 
> tissue type I am imaging.
>
> Has anybody tried linear unmixing using the 488nm laser and produced good 
> images? Is there anything I can do to acquire better images?
>
> Any help would be very much appreciated, thanks!
>   
different tissues might/will have different profiles. have you tuned it
for each tissue type?

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Johan Henriksson
MSc Engineering
PhD student, Karolinska Institutet
http://mahogny.areta.org http://www.endrov.net