You really should make good reference spectra from the different samples
you use: so take samples without fluorescence and make a background
autofluorescence spectrum for each tissue type.



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of samrina aslam
Sent: donderdag 13 november 2008 13:12
To: [log in to unmask]
Subject: Linear unmixing


Hi,

I am currently using linear unmixing on the the Zeiss 510 Meta confocal 
microscope to unmix the background autofluorescence from specific 
fluorescence in parrafin sections.

I am using the 488nm laser, and the results are very varied depending on
the 
tissue type I am imaging.

Has anybody tried linear unmixing using the 488nm laser and produced
good 
images? Is there anything I can do to acquire better images?

Any help would be very much appreciated, thanks!
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