Hi Jon, the pollen grains will fluoresce nicely and are cheap and easy
to make. You can get plastic fluorescent slides from Chroma for free
which are good for maintenance/QC. In my opinion the Molecular Probes
slides are expensive, but well worth it. I have imaged that slide a
thousand times! It always gets a "wow" from the trainee. Pollen grains
are boring! (unless maybe you explode them with your 2P laser).
We have a Zeiss LSM310 Confocal for sale! Let me know if you want to
discuss the particulars.
Cheers,
Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag
ing/Pages/default.aspx
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Jonathan M Krupp
Sent: Friday, November 14, 2008 8:46 AM
To: [log in to unmask]
Subject: Practice samples
Hi:
This is a pretty basic and simple question. I need some
advice about rounding up some samples to use in an
elementary light microscopy class that includes basic
fluorescence.
I am at a community college and my background is in EM and
brightfield LM. I have a passing familiarity with confocal
et al but not enough to know much. I maintained a
confocal, but never ran experiments or prepared much in
the way of samples. Now I have to get something together
to demonstrate the fundamentals of fluorescence to
students in a new job.
I have been relying on chlorophyll autofluorescence up til
now, but would like to add anything that would be easy to
do. We have a simple scope with filters for FITC,
rhodamine, and DAPI, I think. What would be some fool
proof, easy to get samples to try?
In addition, I would eventually like to add some kind of
confocal experience to this class, any ideas about where
to find an inexpensive system would be great.
Thanks
Jon
San Joaquin Delta College
Stockton, CA 95207
[log in to unmask]
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