Jon
You may find the confocal/imaging class lectures I give here at Purdue 
useful for your course. You can find the class lectures at
http://www.cyto.purdue.edu/class/bms524/index.htm

and more at
http://www.cyto.purdue.edu/flowcyt/educate/pptslide.htm

some were recorded using Adobe connect
all have the original PPTs there to download which you may find useful. 
We also have a practical class - if you would like that materials you 
could email me directly

kind regards

paul



Jonathan M Krupp wrote:
> Hi:
> 
> This is a pretty basic and simple question. I need some advice about 
> rounding up some samples to use in an elementary light microscopy class 
> that includes basic fluorescence.
> 
> I am at a community college and my background is in EM and brightfield 
> LM. I have a passing familiarity with confocal et al but not enough to 
> know much. I maintained a confocal, but never ran experiments or 
> prepared much in the way of samples. Now I have to get something 
> together to demonstrate the fundamentals of fluorescence to students in 
> a new job.
> 
> I have been relying on chlorophyll autofluorescence up til now, but 
> would like to add anything that would be easy to do. We have a simple 
> scope with filters for FITC, rhodamine, and DAPI, I think. What would be 
> some fool proof, easy to get samples to try?
> 
> In addition, I would eventually like to add some kind of confocal 
> experience to this class, any ideas about where to find an inexpensive 
> system would be great.
> 
> Thanks
> 
> Jon
> San Joaquin Delta College
> Stockton, CA 95207
> [log in to unmask]


-- 
J. Paul Robinson
SVM Professor of Cytomics
Professor of Immunopharmacology & Biomedical Engineering
Director, Purdue University Cytometry Laboratories
Past-President, International Society for Analytical Cytology
(now International Society for Advancement of Cytometry)

Purdue University Cytometry Laboratories
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