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February 2009

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Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 5 Feb 2009 01:20:56 -0800
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Confocal Microscopy List <[log in to unmask]>
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Yuri Gaidoukevitch <[log in to unmask]>
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Jean-Pierre CLAMME-2 wrote:
> 
> When you do colocalization measurement (in image pro for example) between 
> let say the red and a green channel the co-localization coefficient tells 
> you how much red and green are co-localized.  But as much as I understood, 
> it doesn't give you information on the fact that for example red is always 
> co-localized with green but green is not always colocalized with red ? 
> What kind of analysis  would I need to compare this between samples ? 
> 

Jean-Pierre,

Image-Pro provides this information in m1/m2 and M1/M2 coefficients. You can
find detailed information in
http://www.mediacy.com/pdfs/colocfluorprobes.pdf   

Regards,

 

Yuri Gaidoukevitch
Senior Software Engineer

Media Cybernetics
Frambozenweg 139
2321KA Leiden

The Netherlands
tel  +31-71-5730639 
mobile  +31-621580163 
fax +31-71-5730640

 


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