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February 2009

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Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 19 Feb 2009 11:10:12 -0500
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Confocal Microscopy List <[log in to unmask]>
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Vasseur Monique <[log in to unmask]>
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Dear all,

My question is: 
On the same microscope, same objective, same immersion medium and same
sample, is there a difference in resolution depending of the microscopy
method I am using (fluorescence, luminescence or brightfield) since the
lightpath is not the same?

Is it correct to consider the following?

For brightfield:  r = (1.22 * illumination wavelenght)/(NA objective +
NA condenser)
For fluorescence and confocal: r = (0.61 * excitation (?) wavelenght) /
NA objective
For luminescence: r = (0.61 * emission (?) wavelenght) / NA objective

Thanks in advance,

monique

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