Dear all,
My question is:
On the same microscope, same objective, same immersion medium and same
sample, is there a difference in resolution depending of the microscopy
method I am using (fluorescence, luminescence or brightfield) since the
lightpath is not the same?
Is it correct to consider the following?
For brightfield: r = (1.22 * illumination wavelenght)/(NA objective +
NA condenser)
For fluorescence and confocal: r = (0.61 * excitation (?) wavelenght) /
NA objective
For luminescence: r = (0.61 * emission (?) wavelenght) / NA objective
Thanks in advance,
monique