CONFOCALMICROSCOPY Archives

February 2009

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Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 20 Feb 2009 12:37:26 +0100
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Subject:
Re: Resolution for fluorescence, brightfield and luminescence
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Universität Göttingen
From:
Arwed Weigel <[log in to unmask]>
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All these formulas for confocal microscope resolution assume a 
homogeneously illuminated back-aperture, which is not always the case in 
commercially available microscopes due to the Gaussian intensity profile 
of the excitation laser beam. Therefore, the lateral resolution of a 
confocal microscope can even be worse than of a widefield fluorescence 
microscope with arc-lamp illumination.

-- 
arwed weigel
abteilung fuer neurophysiology und zellulaere biophysik
universitaet goettingen
humboldtallee 23
37073 goettingen

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