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February 2009

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Confocal Microscopy List <[log in to unmask]>
Subject:
poor dapi images on LSM 510
From:
Carl Boswell <[log in to unmask]>
Date:
Fri, 27 Feb 2009 16:19:23 -0700
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Confocal Microscopy List <[log in to unmask]>
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We have been having a chronic problem with the image quality (S/N, 
resolution) of DAPI stained nuclei using a Zeiss 510 Meta.  The quality 
seems to decay over time (months) and pinhole/collimator tuning doesn't make 
things right.  We have had the AOM replaced once but the problem eventually 
returned.  We now have to use 100% 405 laser, rather than 10% to get 
anything close to good images.  Is there something about the AOM stability 
in this light path, or alignment issues with the laser that others have 
found to result in a slow decay in usefulness of this channel?

Thanks for your thoughts.
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX 520-621-3709

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