Dear,

I would advice fluorescence microscopy users to work in a dark room  
not to protect their samples but to be adapted to low light levels if  
you like to see fluorescent cells at low light levels through the  
binocular.

An other option is the use of specific red light as is used in  
photographic dark rooms. However if all is covered and you only have a  
digital image transmitted over the internet, a dark room is not  
necessary, you than can even use the microscope of your neighborgh

Bye

Patrick Van Oostveldt


Quoting Guy Cox <[log in to unmask]>:

> Erik,
>
>        You cannot in any circumstances work with
> multi-photon scopes with the light on.  Sure, 3 confocal
> or widefield scopes in one room can work with no curtains
> (actually we have 5) with local control of lights, but
> NOT multiphoton scopes.
>
>                                               Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>     http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176     Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
>      http://www.guycox.net
> -----Original Message-----
> From: Confocal Microscopy List   
> [mailto:[log in to unmask]] On Behalf Of Erik Manders
> Sent: Saturday, 7 February 2009 10:14 PM
> To: [log in to unmask]
> Subject: Re: Multi-user Imaging room plans?
>
> Just some tips:
> On each microscope we have a Oko-lab incubator with black panels   
> (this Italian company calls the "obscuring panels"; these incubators  
>  are very stable and dedicated for the research environment). So, no  
>  curtains in our microscopy rooms (3 mics per room). And we can work  
>  with the light on!!
> We will move to a new building this year. Between two microscope   
> rooms we will have a small extra room where we will put all the   
> equipment that produces heat and/or noise. Long fibers between the   
> microscopes and laser room. This laser room will be temperature   
> controled (some extra cooling units). So, no warm, noisy, dark room   
> with dusty curtains! Let the sunshine in!
> Good luck, Erik Manders
>
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