the Yoko head can be ordered with a choice of disk to match your  
primary lens.
I was told by Zess the licensing agreement restricts them to selling  
the Yokogawa head coupled to an inverted stand with a stage enclosure  
environmental chamber.


Regards,
Glen
On Feb 10, 2009, at 11:10 AM, Fred Mast wrote:

> Does the CSU-X1 allow you to change the disks? I'm still waiting for  
> a spinning-disk unit that is actually designed for live-cell  
> imaging. The previous Yokogawa units were optimized for 100x 1,4 NA  
> oil objectives if I'm not mistaken. Even with relatively thin  
> samples (cultured cells), you're still introducing spherical  
> aberration...
> By comparison, has anyone else looked at the technology being  
> developed in Raphael Yuste's laboratory? They have combined  
> multiphoton LSM with spatial light modulation technology to  
> effectively "scan" an entire field at once. I'm not an expert in  
> this but in my opinion if you could make it fast enough this seems  
> like a better option to me (they report 60Hz calcium ion imaging).  
> You can check it out here (under the SLM Microscopy section):
>
> http://www.columbia.edu/cu/biology/faculty/yuste/methods.html
>
> Cheers,
>
> Fred
>
> On 10-Feb-09, at 10:55 AM, G. Esteban Fernandez wrote:
>
>> I too was intrigued when I saw Zeiss roll out a spinning disk.   
>> We're happy with our 5 LIVE.  Being able to vary the slit aperture  
>> size to match different objectives/samples is important in our  
>> multi-user facility.  It's also nice to capture two fluorescent  
>> channels simultaneously.  I wish the detector on the 5 LIVE were EM- 
>> CCD but the regular CCD does the job.  Images sometimes have  
>> vertical line artifacts but those are nicely dealt with by Fourier  
>> filtering or dividing by a blank image.  Must be lower cost (if  
>> that's true) and/or established familiarity of the spinning disk  
>> that makes them easy to sell.
>>
>> -Esteban
>>
>> On Tue, Feb 10, 2009 at 10:35 AM, Simon Walker <[log in to unmask] 
>> > wrote:
>> I was interested to see that Zeiss have gone into partnership with  
>> Yokogawa
>> to launch their own flavour of the spinning disk confocal:
>> http://www.microscopy-analysis.com/news/carl-zeiss-and-yokogawa-launch-
>> spinning-disk-cell-observer-sd-2008
>>
>> Does this represent an acknowledgment that the 5-live doesn't do  
>> everything
>> it says on the tin, or just that it's too expensive?  Are there any  
>> happy 5-live
>> users out there?
>>
>>
>>
>> -- 
>> G. Esteban Fernandez, Ph.D.
>> Associate Director
>> Molecular Cytology Core Facility
>> University of Missouri
>> 120 Bond Life Sciences Center
>> Columbia, MO  65211
>>
>> http://www.biotech.missouri.edu/mcc/
>>
>> 573-882-4895
>> 573-884-9395 fax
>
> Fred D. Mast
> Department of Cell Biology
> Medical Sciences Building Room 5-14
> University of Alberta
> Edmonton, Alberta, T6G 2H7
> Canada
>
> Tel: 1-780-492-7407
> [log in to unmask]
>
>
>