TDE is excellent with fluorescence - it also acts as an antifade. You
can get exactly the RI you want by adjusting the amount of water in it.
I've used it for the highest resolution micrographs I ever took. The
one problem is that there are some security restrictions on supplying
it since it is a precursor for mustard gas. But that just means you
may have to sign some forms. Do check out the reference Stanislav
sent because it really does seem like the solution to your problem.
From your previous message, it seems likely that Vectashield is probably
not actually the RI you reported, if all is OK in oil.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Michal Gdula
Sent: Tuesday, 7 April 2009 1:01 AM
To: [log in to unmask]
Subject: Re: Correction of Z-axis distortion- request for opinion
Thank you for your advice!
The price seems to be cheap, but I wander how it works with fluorescence. At the moment probably I will stay with my vectashield so as to not chang to many things in one go.
Regards,
Michal Gdula
Subject: Re: Correction of Z-axis distortion- request for opinion
From: Stanislav Vitha <[log in to unmask]>
Reply-To: Confocal Microscopy List
<[log in to unmask]>
Date: Sat, 4 Apr 2009 03:58:51 -0500
Content-Type: text/plain
I would also consider switching to a mounting medium with correct refractive index (=same RI as immersion oil) 2,2-thiodiethanol comes to mind. See Staudt, 2007. A 500 ml bottle can be had for ~$30.
1. Staudt, T., M.C. Lang, R. Medda, J. Engelhardt, and S.W. Hell, 2,2'-
thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy. Microsc Res Tech, 2007. 70(1): p. 1-9.
Stan Vitha
Microscopy and Imaging Center
Texas A&M University
No virus found in this incoming message.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.11.43/2043 - Release Date: 6/04/2009 6:22 AM
No virus found in this outgoing message.
Checked by AVG.
Version: 7.5.557 / Virus Database: 270.11.43/2043 - Release Date: 6/04/2009 6:22 AM
|
