Howdy,
 
You could try polyester wax. It is a bit trickier to cut than paraffin since it has to be kept cold otherwise it will be too soft to cut. you will also need to float the sections onto ice cold water instead of warm.
 
In my experience i have not seen autofluorecnce due to paraffin wax, usually it is due to the tissue type used.
 
Cheers
 
 
Cam
 
 
 
Cameron J. Nowell
Microscpy Manager
Central Resource for Advanced Microscopy
Ludwig Insttue for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
 
Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104
 
http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
 

________________________________

From: Confocal Microscopy List on behalf of samrina aslam
Sent: Thu 16/04/2009 6:30 PM
To: [log in to unmask]
Subject: embedding medium



Hi,

Does anyone know of an embedding medium which can be used in place of
paraffin, to reduce the autofluoresence produced?

Is there any literature available related to this topic?

Thanks.