Dear Confocalists,

I am trying to locate some commercial and easy-to-use long lifetime
fluorphores to time-gate autofluorescence. Ideally they would have to
last at least 1ms (which is a long time).

The research team I work with makes its own lanthanides organometallic
fluorophores Eu3+ and Tb3+ which last about 3ms. But I am anticipating
getting specimens from collaborators who may want to label the
specimens at their end and lanthanides are not easy to use.

I thought I might be able to find some organometallic transition
metal-ligand complexes such as ruthenium (Ru II), rhenium (Re I), or
osmium (Os II) (10 ns to 10 μs) or some organic fluorophores such as
Pyrene (400ns) or Coronenes (200ns). But I have had no luck finding
any, they might not be easy to use and 400ns is cutting it a bit
short.

One suggestion I have had so far is quantum dots which I need to look
into. There maybe other options I am not aware of.

I would be grateful if you have any suggestions?

Regards,

--
Tom Lawson
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PhD Student,
Macquarie University
NSW, 2109
Australia