Tom,

Although I have no personal experience, in our lab Rhuthenium dyes  
have been used. We also gate the fluorescence and find we can use  
these compounds with lifetimes around 500-800ns. We used them for  
oxygen sensing in cell cultures, so there are protocols to introduce  
them into cells and dark toxicity studies.

e.g. Ru(bipy), RuDPP and Ru(phen). Several compounds are available from Sigma

Sonja

Citat af Tom Lawson <[log in to unmask]>:

> Dear Confocalists,
>
> I am trying to locate some commercial and easy-to-use long lifetime
> fluorphores to time-gate autofluorescence. Ideally they would have to
> last at least 1ms (which is a long time).
>
> The research team I work with makes its own lanthanides organometallic
> fluorophores Eu3+ and Tb3+ which last about 3ms. But I am anticipating
> getting specimens from collaborators who may want to label the
> specimens at their end and lanthanides are not easy to use.
>
> I thought I might be able to find some organometallic transition
> metal-ligand complexes such as ruthenium (Ru II), rhenium (Re I), or
> osmium (Os II) (10 ns to 10 μs) or some organic fluorophores such as
> Pyrene (400ns) or Coronenes (200ns). But I have had no luck finding
> any, they might not be easy to use and 400ns is cutting it a bit
> short.
>
> One suggestion I have had so far is quantum dots which I need to look
> into. There maybe other options I am not aware of.
>
> I would be grateful if you have any suggestions?
>
> Regards,
>
> --
> Tom Lawson
> [log in to unmask]
> PhD Student,
> Macquarie University
> NSW, 2109
> Australia
>