Howdy List,

Has anyone out there had any experience with imaging multiple
fluorescent constructs in the one sample using two photon microscopy? I
have a user that already has a zebrafish with GFP and DsRed in it and
would like to add a third fluorophore if possible. 

Does anyone know if this is possible to image, or have any ideas on a
good choice for the third fluorophore?

A blue protein like CFP would be ideal as its 2PE is ~800nm, leaving GFP
at 920nm and DsRed at ~980nm. But my experience in the past with CFP
under single photon and widefiled fluorescence has been that it is quite
dim. Has anyone used CFP under 2PE before?


Any advice would be greatly appreciated.


Thanks


Cam



Cameron J. Nowell
Microscopy Manager 
Centre for Advanced Microscopy 
Ludwig Institute for Cancer Research 
PO Box 2008 
Royal Melbourne Hospital 
Victoria, 3050 
AUSTRALIA 
Office: +61 3 9341 3155 
Mobile: +61422882700 
Fax: +61 3 9341 3104 
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