Kris --

     Barring any success in the Physiology Department, you might also  
want to check with any C. Elegans labs at your institution.  RNAi by  
injection is a relatively common technique in these labs, and they  
often pull their own injection pipettes using a similar setup.

Best of luck,

Shawn

Shawn Galdeen, Ph.D.
Research Support Specialist
Bio-Imaging Resource Center
Rockefeller University
1230 York Ave.
New York, New York  10065
212.327.7487





Martin Wessendorf wrote:
> Dear Kris--
> Rather than try to buy what you need, you might just go over to the  
> Physiology Department in the medical school and ask who the  
> electrophysiologists are.  An electrophysiologist will have devices  
> that can pull pipettes to a wide range of diameters.  My guess is  
> that you'll want to find someone pulling patch-clamp electrodes-- 
> those tend to be about 1 micron--and then see what you can do by  
> manipulating the pulling parameters.  Or even easier, just take a  
> too-small pipette and bump it under microscopic control against a  
> piece of glass--you can break the tip to just about anything you need.
> Good luck!
> Martin Wessendorf
> [log in to unmask] wrote:
>> Hello everyone,
>>
>> I want to microinject human adipose stem cells with microbeads that  
>> range in size from 0.5-2.8 microns in diameter.  I am using a  
>> Narishige IM-9B microinjector.  This company does not sell pre- 
>> fabricated pipettes.  Can anyone recommend a company/brand of  
>> microinjection pipettes that are useful for microinjection of  
>> microbeads?  Thanks for any advice you can offer.
>>
>> Kris Ford
>> Graduate Student
>> Biomedical Engineering
>> University of North Carolina, Chapel Hill
>> Phone# 919-962-3296