Craig, Perhaps I worded my question incorrectly. I meant to ask about the
currently best achievable level of multiplexing.
Badri Roysam
Professor, Department of Electrical, Computer and Systems Engineering
Associate Director, NSF Center for Subsurface Sensing & Imaging Systems (CenSSIS ERC)
Co-Director, Rensselaer Center for Open Source Software
Rensselaer Polytechnic Institute
110 8th Street, Troy, New York 12180-3590, USA.
Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308): 518-276-8207, Fax: 518-276-8715
Email: [log in to unmask], Web: http://www.ecse.rpi.edu/~roysam
----- Original Message -----
From: Craig Brideau [mailto:[log in to unmask]]
To: [log in to unmask]
Subject: Re: Spectral: Single channel vs Multi-channel
> The Nikon C1Si will do that with spectral unmixing. Speed is about ~1fps
> @512x512 pixels.
>
> Craig
>
>
> On Mon, Apr 13, 2009 at 6:55 AM, Badri Roysam <[log in to unmask]> wrote:
>
> > While on this topic, I am always to eager to learn about ways to image
> many
> > more
> > channels at a time. Has anyone made any breakthroughs lately that may
> > permit us to
> > stain and image, say 10 - 20 channels...?
> >
> >
> > Badri Roysam
> > Professor, Department of Electrical, Computer and Systems Engineering
> > Associate Director, NSF Center for Subsurface Sensing & Imaging Systems
> > (CenSSIS ERC)
> > Co-Director, Rensselaer Center for Open Source Software
> > Rensselaer Polytechnic Institute
> > 110 8th Street, Troy, New York 12180-3590, USA.
> > Office(JEC 7010): 518-276-8067, Assistant: 518-276-8525, Lab(JEC 6308):
> > 518-276-8207, Fax: 518-276-8715
> > Email: [log in to unmask], Web:
> http://www.ecse.rpi.edu/~roysam<http://www.ecse.rpi.edu/%7Eroysam>
> >
> >
> >
> > ----- Original Message -----
> > From: Richard Cole [mailto:[log in to unmask]]
> > To: [log in to unmask]
> > Subject: Spectral: Single channel vs Multi-channel
> >
> >
> > > I looked very carefully at both systems and my conclusion are:
> > >
> > > If you need speed a single channel, a multi-channel spectral PMT is the
> > way
> > > to go.
> > >
> > > If speed is not an issue, as I my case, the scanning system offers a
> > couple
> > > of major advantages
> > >
> > > 1. For spectral unmixing the dyes can have greatly different fluorescent
> > > levels (I have separated two over lapping fluorophores with 10X
> > > concentration difference)
> > >
> > > 2. The size of the slit and the number of sampling points are user
> > > adjustable.
> > >
> > > Just my 2c
> > >
> > >
> > > Rich
> > >
> > > Richard Cole
> > > Research Scientist V
> > > Director: Advanced Light Microscopy & Image Analysis Core
> > > Wadsworth Center
> > > P.O. Box 509 Albany N.Y. 12201-0509
> > >
> > > Research Assistant Professor
> > > Dept. of Biomedical Sciences
> > > School of Public Health State University of New York Albany, New York
> > >
> > > SS518-474-7048
> > > { n518-474-4430
> > > [log in to unmask]
> > > Website www.wadsworth.org/cores/alm/index.htm
> > >
> > >
> > > ________________________________________
> > > From: Confocal Microscopy List [mailto:[log in to unmask]]
> > On
> > > Behalf Of CONFOCALMICROSCOPY automatic digest system
> > > Sent: Sunday, April 12, 2009 1:05 AM
> > > To: [log in to unmask]
> > > Subject: CONFOCALMICROSCOPY Digest - 10 Apr 2009 to 11 Apr 2009
> > (#2009-72)
> > >
> > >
> > >
> > >
> > >
> > > CONFOCALMICROSCOPY Digest - 10 Apr 2009 to 11 Apr 2009 (#2009-72)
> > > Table of contents:
> > > * Spectral: Single channel vs Multi-channel
> > > 1. Spectral: Single channel vs Multi-channel
> > > o Spectral: Single channel vs Multi-channel (04/11)
> > > From: "Richard E. Edelmann" <[log in to unmask]>
> > >
> > >
> > > Browse the CONFOCALMICROSCOPY online archives.
> > >
> > >
> > >
> > >
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