Hi everyone

Thanks for your answers! The ImageJ image5D plugin worked very well. I could
open 20 double channel acquisitions at once, adjust the display for each
channel and save the overlay!

Since it took me a little while to figure out, for the sake of those who
need this feature, here is what I did:
Download the Image5D plugin
Click plugin/Image5D/open series as image5D
Select first image in folder (I had 20 images for 10 cells imaged in 2
channels) and click open then OK
3rd dimension was channel and its size was 2
4th dimension was t and its size was 10.
Underneath the color button I selected 'color' and adjusted the contrast for
each cell in each channel then I checked the overlay by selecting 'ovl'
instead of 'color'.
I selected plugin/Image5D/make montage then ticked in 'display channels
only' + 'all time frames' + 'copy contrast and brightness'. I could then
save my stack of overlays or split them into individual overlays.


Thanks list! :)

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240

> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Dale
> Callaham
> Sent: 27 May 2009 17:34
> To: [log in to unmask]
> Subject: Re: overlay/merge TIFF images
> 
> Hi Sylvie,
> 
> ImageJ can do this easily. I assume that you have 8-bit tiffs
> (monochrome, even if it has a color palette assigned. ou may need to
> change to grayscale and change bit depth to 8-bits. Those are also easy
> operations. Then, in ImageJ (use a current version, I have ver 1.42 and
> it works), just open the images (they must be open), and use "Image |
> Color | Merge Channels" and it allows you to assign the open images to
> the R, G, B, and grayscale channels so you can merge red, green, and
> blue + DIC images for example.
> 
> Dale
> 
> Sylvie Le Guyader wrote:
> > Hi everyone
> >
> >
> >
> > Does anyone know of a simple freeware to overlay/merge Tiff images of
> > identical size but eg different channels?
> >
> >
> >
> > I searched the ImageJ wiki for new plugins but have only found the good
old
> > RGB gray merge which is fine when one has only a few images to overlay
(too
> > many clicks required!) and doesn't need to adjust the levels for each
> > channel. I also had a quick look at BioimageXD but it seems that I
cannot
> > open TIFF images that are not stacks.
> >
> >
> >
> > Any advice?
> >
> >
> >
> > Med vänlig hälsning / Best regards
> >
> >
> >
> > Sylvie
> >
> >
> >
> > @@@@@@@@@@@@@@@@@@@@@@@@
> >
> > Sylvie Le Guyader
> >
> > Dept of Biosciences and Nutrition
> >
> > Karolinska Institutet
> >
> > Novum
> >
> > 14157 Huddinge
> >
> > Sweden
> >
> > +46 (0)8 608 9240
> >
> >