You should look up Nyquist sampling in Pawley's book and set your pixel 
spacing to more sensible numbers. That way your stack will be MUCH 
smaller and you won't run out of memory.

This should help, but there does seem to be a memeory leak problem 
somwhere... Vista is pretty awful too.


Mark Cannell

Matiar Jafari wrote:
> Dear Confocal List Serv Users,
>
> I was wondering if you guys could help me with an issue I've ran into
> now TWICE.  When I shoot a 6 micron z stack with pixel settings of
> .029x.029x.086(.09) with averaging of 8 lines at a speed of 8
> 2048x2048 16 bit file after the image is done I can see a 3D render
> fine etc and i see my image after 59 pain staking minutes of waiting i
> go to click save and when i click save the entire program crashes and
> i get the error windows you see attached in this email and uploaded in
> the link below.  Any advice and help would be GREATLY appreciated.
>
> one of the errors is: "Not enough storage is available to process this command."
>
> http://img38.imageshack.us/img38/8689/50043540.jpg
>
> Thank You
> --
> Matiar Jafari
>