Steve, you are right, the SPOT RT uses a LCD.
If one already has to capture 3 images, I am wondering if it would be a
good idea to use a monochrome camera plus three R/G/B filters sitting in
the fluorescence filter turret. The acquisition software can be set up in
a way that it takes three images and changes the filters in between. Not
the fastest solution, but offers high resolution and sensitivity w/o the
need for an additional b/w camera. One just needs a filter turret and
three filters...
Michael
> Just a minor correction for the archives more than anything else. If I'm
> not
> mistaken the SPOT RT slider does not use a Bayer Mosaic filter but an
> LCD RGB color changing filter, so that three shot colour images may be
> taken
> at full chip resolution.
>
> Cheers
> Steve Cody
> 2009/5/12 David Barnes <[log in to unmask]>
>
>> Thanks Brian, and on that note, the newer RGB Sliders from QImaging have
>> three ports, a clear port for most fluro, a clear port w/ IR blocking,
>> and
>> the LCF port. In addition, the Q filter is easily rotatable with respect
>> to
>> the camera body, to eliminate polarization interference. The filter is
>> controlled thru the Q camera, or can be used with other cameras via a
>> CRI
>> control box.
>>
>> dave
>>
>> On Mon, May 11, 2009 at 12:31 PM, Armstrong, Brian
>> <[log in to unmask]>wrote:
>>
>>> Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga EXi
>>> with a CRI Liquid Crystal Tunable Filter. I think both are viable
>>> options. The SPOT system will most likely cost less and I like the SPOT
>>> software which is easy to use and free. The Retiga/CRI system is a
>>> better system in my humble opinion, and we operate this with Image Pro
>>> Plus software.
>>> Cheers,
>>>
>>>
>>> Brian D Armstrong PhD
>>> Light Microscopy Core Manager
>>> Beckman Research Institute
>>> City of Hope
>>> Dept of Neuroscience
>>> 1450 E Duarte Rd
>>> Duarte, CA 91010
>>> 626-256-4673 x62872
>>> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag
>>> ing/Pages/default.aspx
>>> -----Original Message-----
>>> From: Confocal Microscopy List
>>> [mailto:[log in to unmask]]
>>> On Behalf Of Glen MacDonald
>>> Sent: Monday, May 11, 2009 8:59 AM
>>> To: [log in to unmask]
>>> Subject: Re: digital cameras
>>>
>>> Dear Elaine,
>>> Along the same approach, CRI makes tunable filters that can be fitted
>>> to most microscopes to allow RGB brightfield images with any
>>> monochrome camera. then you still have the ability for fluorescence,
>>> provided your camera has a QE curve and sufficient cooling for long
>>> exposures (or you have bright fluorescent labels). Such filters can
>>> be readily controlled by most acquisition software and provide
>>> automatic white balance to set exposure times. With most acquisition
>>> software, you can also set the fluorescent filters for sequential
>>> acquisition of red, green and blue channels of a brightfield image.
>>> Although, white balance may not be automated.
>>>
>>> Regards,
>>> Glen
>>>
>>> On May 10, 2009, at 11:42 PM, Cameron Nowell wrote:
>>>
>>> > Hi Elaine,
>>> >
>>> > I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders
>>> > for the last 4 years. They work great, very sensitive for
>>> > fluorescence and take good quality colour images. Basically you get
>>> > two cameras in one. The software they use is simple and fairly
>>> > intuitive to use. I don't think i have ever had a user say that it
>>> > was difficult to use.
>>> >
>>> >
>>> > Cheers
>>> >
>>> > Cam
>>> >
>>> >
>>> >
>>> > Cameron J. Nowell
>>> > Microscopy Manager
>>> > Centre for Advanced Microscopy
>>> > Ludwig Institute for Cancer Research
>>> > PO Box 2008
>>> > Royal Melbourne Hospital
>>> > Victoria, 3050
>>> > AUSTRALIA
>>> >
>>> > Office: +61 3 9341 3155
>>> > Mobile: +61422882700
>>> > Fax: +61 3 9341 3104
>>> >
>>> > Facility Website
>>> >
>>> >
>>> > From: Confocal Microscopy List
>>> [mailto:[log in to unmask]
>>> > ] On Behalf Of Peter Pitrone
>>> > Sent: Monday, 11 May 2009 4:34 PM
>>> > To: [log in to unmask]
>>> > Subject: Re: digital cameras
>>> >
>>> > Hello Elaine,
>>> >
>>> > I would recommend a SPOT RT Slider, or something similar. It is a
>>> > Monochrome camera with a bayer filter slider that you can remove for
>>> > fluorescence. Monochrome cameras are always better for fluorescence
>>> > (bayer filters always eat a lot of light) with higher Quantum
>>> > Efficiencies.
>>> >
>>> > Pete
>>> >
>>> > On May 8, 2009, at 8:51 PM, Elaine Kunze wrote:
>>> >
>>> > One of our labs is planning to buy a color digital camera for bright-
>>> > field and fluorescence pictures. They are considering the PAXcam2+
>>> > and Infinity X32. Does anyone have any experience with either of
>>> > these two color cameras or any other camera suggestions?
>>> >
>>> > Elaine Kunze
>>> > Cytometry Facility
>>> > Huck Institute of the Life Sciences
>>> > 319 Life Sciences Building
>>> > Penn State University
>>> > University Park, PA 16802
>>> > http://www.huck.psu.edu/facilities/cytometry-up/
>>> > 814-863-2762
>>> >
>>> >
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>>> >
>>> > This communication is intended only for the named recipient and may
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>>>
>>>
>>>
>>> Glen MacDonald
>>> Core for Communication Research
>>> Virginia Merrill Bloedel Hearing Research Center
>>> Box 357923
>>> University of Washington
>>> Seattle, WA 98195-7923 USA
>>> (206) 616-4156
>>> [log in to unmask]
>>>
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>>
>>
>
>
> --
> Stephen H. Cody
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