I think Olympus has now implemented the tuning of the laser and the
compensation within their software. 

Cameron, where did you get these black incubators? I am interested in
getting one done as well.

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14157 Huddinge
Sweden
+46 (0)8 608 9240
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Cameron
> Nowell
> Sent: 14 May 2009 00:28
> To: [log in to unmask]
> Subject: Re: Recommendations for commercial multi-photon system purchase
> 
> Hi Lisa,
> 
> Thought i woudl chip in with my experience as well. We have 2 Olympus MPE
> Systems, one inverted and one upright that share a common Mai Tai Laser
(the
> laser can only be sent to one scope at a time). The upright is used
routinely for
> intravital imaging and has performed flawlessly. We have imaged zebra fish
and
> mice (both live and dead) on it and acheived some spectacular data sets.
> 
> The current configuration has on large 0.95NA water imersion 20x objective
on it,
> but it is easy enough to slip another turret of objectives onto it if
required. Not sure
> if it is true for other system but the olympus system is tuned to work
with the 20x
> objective in multiphoton mode, so putting other objectives on may not
result in
> optimal imaging. For single photon excitation it doesn't matter.
> 
> The software is stable and easy enough to use. Users are usually up and
going
> independently after a coupel of hours of training and practice.
> 
> The only issue i have with it is that you can not communicate directly
with the laser
> via the Olympus software. You can control the laser power via an ND filter
system
> but other functions (such as wave length tuning) are carried out using a
seperate
> software package installed on a laptop plugged into the laser.
> 
> One last thing worth mentioning is that both microscoes are fitted with
black acrylic
> incubators (thanks to Steve Cody for this). They work so well at blocking
out light
> that you can perform MP imaging with the room lights on!
> 
> Also look into what the service provided by the companies are in your
area. Here in
> Melbourne Olympus are probaly one of the best for response and quality of
service.
> There is no point getting the best microsocpe out there if the peopel in
your area
> are not that good at keeping it going.
> 
> 
> Cheers
> 
> 
> Cam
> 
> 
> 
> Cameron J. Nowell
> Microscpy Manager
> Central Resource for Advanced Microscopy
> Ludwig Insttue for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
> 
> Office: +61 3 9341 3155
> Mobile: +61422882700
> Fax: +61 3 9341 3104
> 
> http://www.ludwig.edu.au/branch/research/platform/microscopy.htm
> 
> 
> ________________________________
> 
> From: Confocal Microscopy List on behalf of Cameron, Lisa
> Sent: Thu 14/05/2009 12:04 AM
> To: [log in to unmask]
> Subject: Recommendations for commercial multi-photon system purchase
> 
> 
> 
> I have been investigating commercial multi-photon systems for awhile in
order to
> purchase a system for my Institute's core microscopy facility. Our
interest is to have
> the capability to do intravital imaging on an upright stand, but also be
able to have
> facility users be able to put slides on and use the visible scanner and
detectors. I
> realize this is a tall order for such versatility in one system, but since
it is for a core
> (which needs to bring in revenue), I'm looking for the most flexible
system. Does
> anyone have any suggestions about the most recent systems on the market?
Or
> could you point out factors you think are the most important for making
the decision
> on which company to go with? Please feel free to contact me off line.
> 
> I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus MPE and
> Prairie's system.
> 
> (BTW - my own experience is with widefield and confocal live-cell imaging,
so I
> have not done 2-p myself, but have been learning about it for about a
year)
> 
> Thanks!
> - Lisa
> 
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy
> Dana Farber Cancer Institute
> 44 Binney St.; JF 215
> Boston, MA 02115
> Office phone: 617-582-8824
> Fax: 617-582-8750
> [log in to unmask]
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