It was my understanding that the 25x is actually smaller (at least in
back aperture size) than the older 20x 0.95?
The beam delivery path, optics and objective mount on the LaVision
BioTec are all custom, ie if they don't already cope with the 25x I
suspect they could easily be made to do. Of course without an
objective to test who really knows :)
(Furthermore the sample stage is also custom, allowing easy
accommodation of the extra length)
I would actually like to see a comparison on the LaVision BioTec
system (or other custom system) of the older 20x Olympus objective
with the newer 25x objective. It was my understanding (and I may be
wrong as it was gleaned from interferences from Olympus competitors -
but supported indirectly by the fine print on at least one Olympus
objective brochure) that the Olympus systems could not fill the 20x
objective and hence that objective did not perform as well in laser
scanning application on a standard Olympus stand as it could have. I
think (again I may be wrong) that the customisations on the LaVision
BioTec system largely overcome that limitation. Therefore the
comparison would be interesting as I wonder how much of the
improvements shown in the Olympus marketing material are due to better
filling on the newer objective?
(bit like showing the effect of pre-chirp compensation by using a high
dispersion system as the base-line - makes the pre-chirp compensation
look extremely compelling :)
Regards,
Adrian
On 14/05/2009, at 11:17 PM, Watkins, Simon C wrote:
> the objective is considerably larger than the standard oly
> objectives. both in thread diameter and length so modifications of
> the stand using oly parts is needed too.
>
> Simon C. Watkins Ph.D, FRCPath
> Professor and Vice Chair, Cell Biology and Physiology
> Professor, Immunology
> Director, Center for Biologic Imaging
> BSTS 225, University of Pittsburgh
> 3500 Terrace St.
> Pittsburgh PA 15261
> Tel: 412-352-2277
> Fax:412-648-2797
> URL: http://www.cbi.pitt.edu
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]
> ] On Behalf Of Adrian Smith
> Sent: Thursday, May 14, 2009 7:58 AM
> To: [log in to unmask]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
>
> I don't believe there is any technical reason why the 25x Olympus will
> not work on the LaVision BioTec or Prairie systems.
>
> However, my understanding is that Olympus will only sell that
> objective with a new Olympus MPE system... (which makes it a lot more
> than just "super expensive" :).
>
> Extremely disappointing decision on Olympus' part.
>
> I would absolutely love to try the objective on our Olympus microscope
> (supplied by LaVision BioTec) but I am unable to. If it performs as
> well as the marketing material suggests then I would be willing to pay
> a premium for it.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
> On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:
>
>> If and when you try the olympus system make sure they bring you the
>> super expensive dipping/coverslip water optic (25X1.12 NA) which
>> they designed specifically for 2p. It is absolutely spectacular,
>> and pretty much the only lens we use for all our mpe work nowadays.
>> When you compare systems it may make you lean towards this
>> particular manufacturer. Of course the LaVision and Prairie scan
>> heads will fit on the Oly stand, not sure whether they will work
>> with this lens but it would be great to find out (anyone out there
>> tried this combo yet).
>> S.
>> Simon C. Watkins Ph.D, FRC Path
>> Professor and Vice Chair Cell Biology and Physiology
>> Professor Immunology
>> Director Center for Biologic Imaging
>> BSTS 225
>> University of Pittsburgh
>> 3500 Terrace St
>> Pittsburgh PA 15261
>> 412-352-2277
>> www.cbi.pitt.edu
>>
>> ________________________________________
>> From: Confocal Microscopy List [[log in to unmask]] On
>> Behalf Of Adrian Smith [[log in to unmask]]
>> Sent: Wednesday, May 13, 2009 8:04 PM
>> To: [log in to unmask]
>> Subject: Re: Recommendations for commercial multi-photon system
>> purchase
>>
>> On 14/05/2009, at 1:57 AM, Alison North wrote:
>>
>>> If you were only interested in multiphoton imaging, the LaVision has
>>> certain advantages, but if you need visible lasers too then I'm
>>> afraid it won't suit your purposes.
>>
>> I'm not sure of the details but the LaVision BioTec did mention some
>> confocal options to me in passing when we were discussing
>> specifications recently. We were not interested so I didn't ask any
>> details but it might be worth clarifying with them what they can
>> currently offer.
>>
>> From recollection I think the Prairie was also MP-only, at least when
>> we were looking (that was one of its selling points). Maybe that has
>> changed or I'm misrembering?
>>
>> Regards,
>>
>> Adrian Smith
>> Centenary Institute, Sydney, Australia
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