My information came directly from an Olympus Australia representative.

Regards,

Adrian

On 14/05/2009, at 11:05 PM, Guy Cox wrote:

> This should not be any problem on (for example) a Prairie or  
> LaVision system fitted on an Olympus stand.  I really find it hard  
> to believe that Olympus would not supply this lens for such use.   
> Can anyone actually confirm that they will not?  Is there anyone  
> from Olympus on this list?
>
>                                     Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>    http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176     Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
>     http://www.guycox.net <http://www.guycox.net/>
>
>
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> ________________________________
>
> From: Confocal Microscopy List [mailto:[log in to unmask] 
> ] On Behalf Of RICHARD BURRY
> Sent: Thursday, 14 May 2009 10:47 PM
> To: [log in to unmask]
> Subject: Re: Recommendations for commercial multi-photon system  
> purchase
>
>
> The Olympus 25x 1.05 N.A. objective has an enlarged aperture in the  
> back image plane to allow more light throughput.  This requires  
> additional lenses capable of filling the aperture, which Olympus  
> has.  While the lens may fit on other microscopes, with ut the  
> filling of this aperture, it will not perform as well as it could.
>
> Dick
>
> ----- Original Message -----
> From: Adrian Smith <[log in to unmask]>
> Date: Thursday, May 14, 2009 8:02 am
> Subject: Re: Recommendations for commercial multi-photon system  
> purchase
> To: [log in to unmask]
>
>> I don't believe there is any technical reason why the 25x
>> Olympus will
>> not work on the LaVision BioTec or Prairie systems.
>>
>> However, my understanding is that Olympus will only sell
>> that
>> objective with a new Olympus MPE system... (which makes it a lot
>> more
>> than just "super expensive" :).
>>
>> Extremely disappointing decision on Olympus' part.
>>
>> I would absolutely love to try the objective on our Olympus
>> microscope
>> (supplied by LaVision BioTec) but I am unable to. If it performs
>> as
>> well as the marketing material suggests then I would be willing
>> to pay
>> a premium for it.
>>
>> Regards,
>>
>> Adrian Smith
>> Centenary Institute, Sydney, Australia
>>
>> On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:
>>
>>> If and when you try the olympus system make sure they bring
>> you the
>>> super expensive dipping/coverslip water optic (25X1.12 NA)
>> which
>>> they designed specifically for 2p.  It is absolutely
>> spectacular,
>>> and pretty much the only lens we use for all our mpe work
>> nowadays.
>>> When you compare systems it may make you lean towards
>> this
>>> particular manufacturer.  Of course the LaVision and
>> Prairie scan
>>> heads will fit on the Oly stand, not sure whether they will
>> work
>>> with this lens but it would be great to find out (anyone out
>> there
>>> tried this combo yet).
>>> S.
>>> Simon C. Watkins Ph.D, FRC Path
>>> Professor and Vice Chair Cell Biology and Physiology
>>> Professor Immunology
>>> Director Center for Biologic Imaging
>>> BSTS 225
>>> University of Pittsburgh
>>> 3500 Terrace St
>>> Pittsburgh PA 15261
>>> 412-352-2277
>>> www.cbi.pitt.edu
>>>
>>> ________________________________________
>>> From: Confocal Microscopy List
>> [[log in to unmask]] On
>>> Behalf Of Adrian Smith [[log in to unmask]]
>>> Sent: Wednesday, May 13, 2009 8:04 PM
>>> To: [log in to unmask]
>>> Subject: Re: Recommendations for commercial multi-photon
>> system
>>> purchase
>>>
>>> On 14/05/2009, at 1:57 AM, Alison North wrote:
>>>
>>>> If you were only interested in multiphoton imaging, the
>> LaVision has
>>>> certain advantages, but if you need visible lasers too then I'm
>>>> afraid it won't suit your purposes.
>>>
>>> I'm not sure of the details but the LaVision BioTec did
>> mention some
>>> confocal options to me in passing when we were discussing
>>> specifications recently. We were not interested so I didn't
>> ask any
>>> details but it might be worth clarifying with them what they can
>>> currently offer.
>>>
>>> From recollection I think the Prairie was also MP-only, at
>> least when
>>> we were looking (that was one of its selling points). Maybe
>> that has
>>> changed or I'm misrembering?
>>>
>>> Regards,
>>>
>>> Adrian Smith
>>> Centenary Institute, Sydney, Australia
>>
>>
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>
> Richard W. Burry, Ph.D.
> Department of Neuroscience, College of Medicine
> Campus Microscopy and Imaging Facility, Director
> The Ohio State University
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