Yes - brand "N" is getting a much larger look in from Brand "L" than
in the past.
On 15/05/2009, at 12:15 AM, Guy Cox wrote:
> Well, what can I say? You recently posted that you are in the
> market for a second system. You can just insist that your third-
> party system must be based on 'brand N' or 'brand L' or even 'brand
> Z' if 'brand O' will not give you the assurance of optics supply
> that you need. The same applies to anyone buying a 3rd-party
> system. The marketplace isn't that big - a handful of serious
> orders carries rather substantial weight.
>
> Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]
> ] On Behalf Of Adrian Smith
> Sent: Thursday, 14 May 2009 11:51 PM
> To: [log in to unmask]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
>
> My information came directly from an Olympus Australia representative.
>
> Regards,
>
> Adrian
>
> On 14/05/2009, at 11:05 PM, Guy Cox wrote:
>
>> This should not be any problem on (for example) a Prairie or LaVision
>> system fitted on an Olympus stand. I really find it hard
>> to believe that Olympus would not supply this lens for such use.
>> Can anyone actually confirm that they will not? Is there anyone from
>> Olympus on this list?
>>
>> Guy
>>
>>
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox CRC Press / Taylor & Francis
>> http://www.guycox.com/optical.htm
>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope
>> Unit,
>> Madsen Building F09, University of Sydney, NSW 2006
>> ______________________________________________
>> Phone +61 2 9351 3176 Fax +61 2 9351 7682
>> Mobile 0413 281 861
>> ______________________________________________
>> http://www.guycox.net <http://www.guycox.net/>
>>
>>
>>
>> ________________________________
>>
>> From: Confocal Microscopy List
>> [mailto:[log in to unmask]
>> ] On Behalf Of RICHARD BURRY
>> Sent: Thursday, 14 May 2009 10:47 PM
>> To: [log in to unmask]
>> Subject: Re: Recommendations for commercial multi-photon system
>> purchase
>>
>>
>> The Olympus 25x 1.05 N.A. objective has an enlarged aperture in the
>> back image plane to allow more light throughput. This requires
>> additional lenses capable of filling the aperture, which Olympus has.
>> While the lens may fit on other microscopes, with ut the filling of
>> this aperture, it will not perform as well as it could.
>>
>> Dick
>>
>> ----- Original Message -----
>> From: Adrian Smith <[log in to unmask]>
>> Date: Thursday, May 14, 2009 8:02 am
>> Subject: Re: Recommendations for commercial multi-photon system
>> purchase
>> To: [log in to unmask]
>>
>>> I don't believe there is any technical reason why the 25x Olympus
>>> will not work on the LaVision BioTec or Prairie systems.
>>>
>>> However, my understanding is that Olympus will only sell that
>>> objective with a new Olympus MPE system... (which makes it a lot
>>> more
>>> than just "super expensive" :).
>>>
>>> Extremely disappointing decision on Olympus' part.
>>>
>>> I would absolutely love to try the objective on our Olympus
>>> microscope (supplied by LaVision BioTec) but I am unable to. If it
>>> performs as well as the marketing material suggests then I would be
>>> willing to pay a premium for it.
>>>
>>> Regards,
>>>
>>> Adrian Smith
>>> Centenary Institute, Sydney, Australia
>>>
>>> On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:
>>>
>>>> If and when you try the olympus system make sure they bring
>>> you the
>>>> super expensive dipping/coverslip water optic (25X1.12 NA)
>>> which
>>>> they designed specifically for 2p. It is absolutely
>>> spectacular,
>>>> and pretty much the only lens we use for all our mpe work
>>> nowadays.
>>>> When you compare systems it may make you lean towards
>>> this
>>>> particular manufacturer. Of course the LaVision and
>>> Prairie scan
>>>> heads will fit on the Oly stand, not sure whether they will
>>> work
>>>> with this lens but it would be great to find out (anyone out
>>> there
>>>> tried this combo yet).
>>>> S.
>>>> Simon C. Watkins Ph.D, FRC Path
>>>> Professor and Vice Chair Cell Biology and Physiology Professor
>>>> Immunology Director Center for Biologic Imaging BSTS 225 University
>>>> of Pittsburgh 3500 Terrace St Pittsburgh PA 15261
>>>> 412-352-2277
>>>> www.cbi.pitt.edu
>>>>
>>>> ________________________________________
>>>> From: Confocal Microscopy List
>>> [[log in to unmask]] On
>>>> Behalf Of Adrian Smith [[log in to unmask]]
>>>> Sent: Wednesday, May 13, 2009 8:04 PM
>>>> To: [log in to unmask]
>>>> Subject: Re: Recommendations for commercial multi-photon
>>> system
>>>> purchase
>>>>
>>>> On 14/05/2009, at 1:57 AM, Alison North wrote:
>>>>
>>>>> If you were only interested in multiphoton imaging, the
>>> LaVision has
>>>>> certain advantages, but if you need visible lasers too then I'm
>>>>> afraid it won't suit your purposes.
>>>>
>>>> I'm not sure of the details but the LaVision BioTec did
>>> mention some
>>>> confocal options to me in passing when we were discussing
>>>> specifications recently. We were not interested so I didn't
>>> ask any
>>>> details but it might be worth clarifying with them what they can
>>>> currently offer.
>>>>
>>>> From recollection I think the Prairie was also MP-only, at
>>> least when
>>>> we were looking (that was one of its selling points). Maybe
>>> that has
>>>> changed or I'm misrembering?
>>>>
>>>> Regards,
>>>>
>>>> Adrian Smith
>>>> Centenary Institute, Sydney, Australia
>>>
>>>
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>>
>> Richard W. Burry, Ph.D.
>> Department of Neuroscience, College of Medicine Campus Microscopy and
>> Imaging Facility, Director The Ohio State University Associate
>> Editor,
>> Journal of Histochemistry and Cytochemistry
>> 277 Biomedical Research Tower
>> 460 West Twelfth Avenue
>> Columbus, Ohio 43210
>> Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849
>>
>>
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