You could try double sided tape layered on each other or wax. Grace  
Biolabs also makes imaging chamber spacers (no commercial interest):

http://www.gracebio.com/Products/Imaging__Microscopy/ 
SecureSeal_Imaging_Spacers/

They make spacers with a variety of thicknesses, and I recall using  
one version that was very thin.
John Oreopoulos


On 9-Jul-09, at 3:27 PM, Sam Albers wrote:

> Hello all,
>
> My institution's confocal set up uses an inverted microscope. So  
> when I view
> some biofilm samples I am collecting, I need to also invert my  
> slide and
> place it in a NUNC coverslip chamber. Because I do not want to  
> crush my
> biofilm sample, I need thin spacers to separate the slide from the  
> coverslip
> bottom. Thin glass microscope slide have been suggested as a  
> spacer. I was
> wondering if any one here had any other suggestions.
>
> I am looking for a convenient inert object that can be be easily  
> placed
> inside the coverslip chamber that will separate the slide from the  
> coverslip
> bottom. Ideally, this would some type of material that I could vary  
> the
> length to account for different thicknesses of biofilms.
>
> Any suggestions would be greatly appreciated. I guess I am just  
> wondering
> what other people do.
>
> Thanks!
>
> Sam
> -- 
> View this message in context: http://n2.nabble.com/Spacers-for- 
> Inverted-Microscop-tp3233585p3233585.html
> Sent from the Confocal Microscopy List mailing list archive at  
> Nabble.com.