My favourites are Leica SP5 and Nikon A1R.
All the best
Alby
On Jul 31, 2009, at 10:44 PM, Michael Meade wrote:
>
>
> Sent from my Windows Mobile® phone.
>
> From: Adam Larson <[log in to unmask]>
> Sent: Friday, July 31, 2009 6:33 AM
> To: [log in to unmask]
> <[log in to unmask]>
> Subject: Re: Buying a COnfocal Microscope
>
> The best choice for you will come down to the different approaches
> each of the manufacturers takes to spectral imaging. The Leica and
> Olympus benefit from nearly infinite spectral resolution tuning.
> However, due to their few number of detectors their spectral imaging
> speed is going to be quite slow especially if the spectral spread of
> your nanoparticles is wide. This will only get slower as your
> spectral resolution increases. Among those two, Leica will have the
> most efficient beam path. While I do not believe their claim of
> >90% transmission efficiency on the AOBS, the very narrow filter
> channels for excitation lasers make it a better choice than
> multichannel dichroics. It also uses a prism to refract the light
> instead of a diffraction grating. That being said, if speed is an
> issue, The LSM710, as far as I know, collects 34 channels
> simultaneously. It has a 32 channel PMT array plus two additional
> PMT’s next to the main array that have slits in front of them for
> varying spectral resolution since the main PMT array has a fixed
> channel resolution. An alternative suggestion might be to look at
> the new Nikon A1R. As with the LSM710 is has a grating and
> 32channel PMT array based spectral detector. Unlike the LSM710, the
> A1R spectral detector has a choice of three gratings allowing the
> user to adjust spectral resolution and center wavelength. Both
> Nikon and Zeiss play clever tricks to improve the diffraction
> efficiency of gratings. The Nikon A1R also uses dichroic mirrors
> optimized for a low angle of incidence that significantly narrows
> the laser reflection bands improving transmission efficiency. They
> can not get as narrow as the AOBS from Leica, but are an improvement
> over the traditional 45degree dichroics. The Nikon A1R and the
> Leica SP5 are both tandem scanner arrangements meaning that there is
> a galvo pair and a galvo/resonance pair. Nikon actively corrects
> for pixel shifts between the forward and back scans of the resonance
> scanner, Leica has a look-up table and a user correction in software.
>
> I may not have made your decision any easier, but I hope it at least
> helps.
>
> Regards,
>
> Adam M. Larson, Ph.D.
> Advanced Imaging Group
> 435 Route 206
> Newton, NJ 07860
> Tel: (973)300-4497
>
> THORLABS Inc.
> From: Confocal Microscopy List [mailto:[log in to unmask]
> ] On Behalf Of Manish Kumar
> Sent: Friday, July 31, 2009 12:46 AM
> To: [log in to unmask]
> Subject: Re: Buying a COnfocal Microscope
>
> Hi Amol
> I have used Leica TCS SP5 and Zeiss meta 510, out of these two the
> choice goes for Leica TCS SP5.I think Leica is the best suited for
> your experiments. Leica is the only comp who uses AOBS and this AOBS
> makes Leica different from others.
> All the best
> Manish Kumar
> Senior research associate
> Delhi University, South campus,
> New Delhi.
> India
> +919717379212
>
>
> On Fri, Jul 24, 2009 at 12:38 AM, Amol K <[log in to unmask]> wrote:
> Hello All,
>
> Sorry to bother everyone with these questions but what is a better
> resource
> than confocal users and specialists! I tried searching for similar
> topics but
> didn't get much useful information.
>
> We will be adding confocal microscope to our facility here at my
> company. I've
> three quotes from Olympus, Leica and Carl Zeiss. The models are
> Olympus
> FV1000/IX81, Leica TCS SP5 and Carl Zeiss LSM710. Olympus and Leica
> are
> almost similar in costs whereas Carl Zaeiss is relatively costly for
> similar
> instrumentation.
>
> My experiment will range from cellular uptake studies of
> nanoparticles to some
> FRET experiments. The nanoparticles will be multi colored and hence
> spectral
> unmixing will be important. There will be not many users, perhaps
> 3/4 at max.
>
> My questions are how good are these systems when compared to each
> other,
> which one you will recommend consideing the ease of use, reliability
> and
> service, any specific advantage of one over another.
>
> I really hope you will take some time out and reply to these
> questions. Thank
> you in advance.
>
> Best,
> Amol
>
----------------------------------------------------
"Water slowly flowed under the sky" (Cesare Pavese)
-----------------------------------------------------
Alberto Diaspro,
LAMBS IFOM IEO -MicroSCoBio, NBT-IIT, IBF-CNR
Department of Physics, University of Genoa,
Via Dodecaneso 33, 16146 Genoa, Italy -
fax +39-010314218 - tel +39 0103536426/309;
URLs: www.lambs.it;
Win in Science! ...link to http://www.ebsa2009.org
----------------------------------------------
----------------------------------------------------
"Water slowly flowed under the sky" (Cesare Pavese)
-----------------------------------------------------
Alberto Diaspro,
LAMBS IFOM IEO -MicroSCoBio, NBT-IIT, IBF-CNR
Department of Physics, University of Genoa,
Via Dodecaneso 33, 16146 Genoa, Italy -
fax +39-010314218 - tel +39 0103536426/309;
URLs: www.lambs.it;
Win in Science! ...link to http://www.ebsa2009.org
----------------------------------------------
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