Subject: | |
From: | |
Reply To: | |
Date: | Fri, 10 Jul 2009 13:13:27 -0700 |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
If you are doing tunel you can make your own by treating the sections
with DNase prior to staining......otherwise I might try to find a
decent tumour sample, with a little necrosis.
Happy Friday!
Caroline
Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158
www.gladstone.ucsf.edu
On Jul 10, 2009, at 11:20, "Sobocinski, Gregg" <[log in to unmask]>
wrote:
> Do you have access to lactating rats? I've used post-lactation rat
> mammary glands. I believe the time period was 2-10 days post-
> lactation, but am not positive. Send me a personal message if you
> want me to look up the exact range for harvesting.
>
> Regards,
> ~Gregg
>
> Gregg Sobocinski
> Microscope Imaging Specialist
> University of Michigan, MCDB Dept.
> Ann Arbor, Michigan
> USA
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Martin
> Wessendorf
> Sent: Friday, July 10, 2009 1:58 PM
> To: [log in to unmask]
> Subject: positive control for apoptosis
>
> Dear all--
>
> Can anyone suggest a good tissue to use as a positive control for
> apoptosis? We've considered liver and testes but as nearly as I can
> find from the literature, the proportion of apoptotic cells in those
> organs in naive rats is pretty small. Any other suggestions?
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D. office: (612) 626-0145
> Assoc Prof, Dept Neuroscience lab: (612) 624-2991
> University of Minnesota Preferred FAX: (612) 624-8118
> 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009
> Minneapolis, MN 55455 e-mail: [log in to unmask]
|
|
|