Dear Sir,

I will be interested to see the public replies as the page hyperlinked does 
not give any clear message.

Warm Regards.

Anil

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From: "Raman Rajagopal" <[log in to unmask]>
Sent: Friday, July 03, 2009 4:49 PM
To: <[log in to unmask]>
Subject: Has anybody any experience in using the new Zeiss LSM 700?

> Zeiss has this year come up with a new system LSM 700. They claim it is 
> spectral - by
> varying the position of the second dichroic (VSD) which is an interference 
> filter device.
> The VSD, as I understand, has the ability to dynamically reflect / 
> transmit different
> wavelength along its length thus achieving diffraction of the emission 
> into its spectral
> components with out using a prism or grating.
> (http://zeiss-campus.magnet.fsu.edu/tutorials/spectralimaging/lsm700/index.html 
>  - try
> varying the beam splitter position in the diagram).
>
> 1. How would you rate LSM 700 vis a vis other machines with respect to 
> their inherent
> ability in capturing the spectral data.
> 2. What would be the efficiency of VSD when compared to a prism and 
> grating method in
> spectral dispersion.
> 3. LSM 700 has 4 solid state laser lines coming directly into the machine. 
> They do not
> mention an AOTF, but they mention that we can control individually the 
> laser power
> intensity. Can this be done with out AOTF and how effective will this be?
> 4.. When compared to all other machines, LSM 700 lasers have much less 
> power: 405 - 5
> mW; 488 - 10 mW; 555 - 10 mW and 639 - 5 mW. Does this mean that 700 is 
> more
> sensitive or it is a trade off for a lesser price that they offer.
>
>
>
>
> RAJAGOPAL  RAMAN
> Dept. of Zoology,
> University of  Delhi,
> New Delhi. India.
>