Hi Guosheng,

We find the same problem occurs with our Zeiss 510Meta if you turn up 
the power on the argon laser too quickly - it just cuts out.  So, if you 
hit the re-use button while the laser is on standby or minimum power, 
this could certainly cause it to cut out.  Likewise, if you change users 
the power will have switched back to standby when the first one logs 
off.  The critical thing seems to be to turn the laser from standby to 
on, and then to slowly increase the power click by click, rather than 
dragging it up fast with the slider.  I wonder whether you will find you 
solve the problem by turning up the power on the laser BEFORE clicking 
on the re-use button?

Of course, if you've never encountered this problem before, it is more 
likely to be that the laser is simply getting old...

Best,
Alison

G.Liu wrote:
> Hello Listers,
>
> In last few days, the argon laser in our Zeiss 510Meta encountered twice the
> shutdown problem during operation: the readings of laser current became
> -0.2A (output at about 75% position). When dragging back to 25% output, the
> current become normal (4.5A). Those happened, at one time, during "Reuse"ing
> the configuration of an old image; and at other time, during switching
> users. 
>
> Is this problem caused by inappropriate operation or Argon laser's lifespan
> is almost reached, or other reasons? Any advice will be greatly appreciated! 
>
> Guosheng
>   

-- 
Alison J. North, Ph.D.,
Research Assistant Professor and 
Director of the Bio-Imaging Resource Center,
The Rockefeller University,
1230 York Avenue,
New York,
NY 10065.
Tel: office	++ 212 327 7488
Tel: lab    	++ 212 327 7486
Fax:        	++ 212 327 7489