They sell these "test slides" for hundreds of dollars, yet they don't take the time to make them right?!?! They should be made with correct coverslips chosen for their thickness, deposited on them and then mounted on the glass slide... I see that there is a disconnect some where, how much does it cost them to produce these slides? 10 bucks maybe...

Pete

On Mar 26, 2010, at 12:25 PM, Guy Cox wrote:

> No, no, it IS the problem!  As Mike Ignatius explained, MP put the beads on the slide, not the coverslip, then add mountant and then the coverslip.  If you are using a #1.5 coverslip you need to put the beads directly on the coverslip.  With the beads on the slide the extra thickness of the mountant needs to be corrected for by using a thinner coverslip - #1 or #0 - which must be found by trial and error.  (But since I guess they are using very reproducible conditions they only need to do the test once).  At least it seems they have realized they have a problem.  
> 
>                                       Guy
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of MICROSCOPIA CONFOCAL y CCD
> Sent: Thursday, 25 March 2010 10:15 PM
> To: [log in to unmask]
> Subject: Re: FocalCheck test slide
> 
>> Thanks a lot for your answers.
> 
>> Of course I put the slide upside down when using 
>> inverted microscope and I asked for Molecular 
>> probes about coverslip and they answered me: 
>> "The coverslip thickness for the FocalCheck 
>> prepared slides is 0.13-0.16 mm" It´s no ideal 
>> but I think that this is not the problem.
> 
> M. Teresa
> 
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