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March 2010

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Subject:
Best FRET pair
From:
Maria Calvo <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 26 Mar 2010 16:48:24 +0100
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Dear all,

We are planning to do FRET experiments with a fluorescent protein (YFP, mCherry or mOrange)and a directly labelled primary antibody. 

We are restricted to YFP, mCherry or mOrange for one of the proteins (the most abundant). 

Does anyone know what's the best FRET pair for any of these FPs, in terms of J (overlap), brightness ?

Thanks for your help,

Maria Calvo

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___________________________________

Dra. Maria Calvo

Unitat de Microscòpia Confocal
Serveis Cientificotècnics-C.Casanova
Facultat de Medicina
Universitat de Barcelona- IDIBAPS
C/ Casanova 143
Barcelona 08036

Tel: 34 934037159/39930
Fax: 34 934039946
E-mail: [log in to unmask]
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